DNA

Part:BBa_K3696016

Designed by: Yuanhong Pan   Group: iGEM20_OSA   (2020-10-26)
Revision as of 18:52, 26 October 2020 by Hpan20 (Talk | contribs)

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A type of DNA oligonucleotide that can cleave towards its substrate only after binding to Mn2+.

BBa_K3696016 is used in S9 and Cas12a combination testings, the protocol is shown below:

S9 protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37 degree.

S9&Cas12a combined testing

From the detected fluorescence, we determine that: S9 is suitable for Cas12a binding.

S9 has specificity for Mn2+.

S9 specificity testing


S9's detection limit for Mn2+ (100 μM).

S9 Mn2+ concentration testing


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None