DNA

Part:BBa_K3696019

Designed by: Chaohan Ji   Group: iGEM20_OSA   (2020-10-25)
Revision as of 18:27, 26 October 2020 by OscarJi (Talk | contribs)

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A mutant of BBa_K3696000.

BBa_K3696019--BBa_K3696113, BBa_K3696002, BBa_K3696003 are used in 8-17 DNAzyme mutation testings, the protocol is shown below:

8-17 DNAzyme mutation test protocol


The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

8-17 DNAzyme mutation testing


From the detected fluorescence, we determine that 8-17 DNAzyme mut 10 has higher activity and same detection limit as the original 8-17 DNAzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None