Coding

Part:BBa_K3396007

Designed by: yingqian Ye   Group: iGEM20_NUDT_CHINA   (2020-10-24)
Revision as of 18:14, 26 October 2020 by Qiuxinyuan12 (Talk | contribs)


Truncated Trim21

This part is the truncated version of human Trim21 coding sequence. We removed the PRYSPRY domain containing 268-475 aa of the original Trim21 to allow further engineering.

Usage and Biology

BBa_K3396007 is the core of the newly registered Predator Pro system. Trim 21 comes from the family of TRIM. We truncated the Trim21 protein to maintain its RING domain, B box domain and coiled coli domain, while the antibody binding PRYSPRY domain was removed. This truncate protein maintained the E3 ubiquitin ligase activity, and provided an open interface for other protein dimerization pairs to be added. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None