DNA

Part:BBa_K3696015

Designed by: Yuanhong Pan   Group: iGEM20_OSA   (2020-10-26)
Revision as of 17:59, 26 October 2020 by Hpan20 (Talk | contribs)


A type of DNA oligonucleotide that can cleave towards its substrate only after binding to Pb2+.

BBa_K3696015 is used in GR-5 and Cas12a combination testings, the protocol is shown below:

GR-5 protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

GR-5&Cas12a combined testing

From the detected fluorescence, we determine that: GR-5 is not suitable for Cas12a binding.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None