Coding

Part:BBa_K3595002

Designed by: Ying Li   Group: iGEM20_GZ_HFI   (2020-10-26)
Revision as of 17:29, 26 October 2020 by Registry (Talk | contribs)

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Mutant argA-fbr

ArgA gene takes part in the first step that converts glutamate into arginine. The function of the synthetase encoded by ArgA is influenced by the concentration of arginine. The higher the concentration, the lower effect the enzyme will have.However, the arginine synthesis pathway is repressed through two regulatory pathways.One of them is arginine itself has feedback inhibition on NAGS. In order to maximize the conversion of ammonia to arginine, feedback-resistant (fbr) NAGS enzymes is required, whose argA gene is mutated into argA^fbr by substituting the sequences TAT (Tyr) at 19th position into TGT (Cys), and the GCG (Ala) at 389th position into GCT (Ala).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 626


[edit]
Categories
//cds
Parameters
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