Composite

Part:BBa_K3482035

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-23)
Revision as of 17:04, 26 October 2020 by Santosmoreno j (Talk | contribs) (→‎Usage and Biology)


azurin with pelB secretion and 8His purification tag

Azurin fused with a 8His tag and with a pelB secretion signal peptide for protein expression and purification

Usage and Biology

Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None