Coding

Part:BBa_K3599036

Designed by: Zijia Liu   Group: iGEM20_TPR_China   (2020-10-24)
Revision as of 17:03, 26 October 2020 by Eloiseliu (Talk | contribs)


paAIMT

An enzyme which was designed into the artificial biosynthesis pathway of 4VA synthesis. The original function of paAIMT was converting t-anol or isoeugenol to t-anethole or methylisoeugenol, respectively, via methylation of the para-OH group. The codon usage was optimized for expression in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 979
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 979
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 979
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 979
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None