Composite

Part:BBa_K3482018

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-19)
Revision as of 17:01, 26 October 2020 by Santosmoreno j (Talk | contribs) (→‎Usage and Biology)


IPTG and heat-repressible miniColicin toxin

MiniColicin E2 version regulated by a heat-repressible termosensor and activited by IPTG under a pTac promotor

Usage and Biology

Colicin E2 is an endonuclease that cuts on both single and double-stranded DNA. It has no specific cutting site. Colicins are toxins that can be produced by E.coli and closely related bacteria.

We will use this part in a Kill-switch with the IM2 protein, which provides immunity to Colicin E2.

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482017 .


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
    Illegal AgeI site found at 155
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None