Part:BBa_K3467003:Experience

In our project, we designed three basic part, targeting to three different genes of locust. In our design, we selected Locust migratoria gene trypsin-like serine protease, chitin synthase and ATPase subunitH gene as our target gene. By using specific program to design dsRNA sequence, we finally designed our three basic parts: TSP dsRNA sequence, CHS dsRNA sequence and ATPase dsRNA sequence.

We also measured their effect to locust by injection experiment.

As the result shows:

https://2020.igem.org/File:T--CAU_China--pw5.jpg

Comparing with the WT and control group (by injecting DEPC water) locust survival rate, we used LT50 to measure dsRNA effect. From the diagram above, CHS dsRNA injection has a great effect to locust which CHS experiment group LT50 is approximately 4days.

https://2020.igem.org/File:T--CAU_China--pw6.jpg

Here is the survival rate that we measure during the whole experiment process.