Part:BBa_K3407004:Design
Fox-1 RBD: a protein domain binding strongly and specifically to RNA sequence UGCAUGU.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Fox-1 amino acid sequence retrieved from NCBI residues 109–208 following the Literature’s materials and methods. As an N-HisTag from pET-28(a) was used in materials and methods, its amino acid sequence was added in the N-Terminal of Fox-1. The Cry7Ca1 gene was codon-optimized for expression in Escherichia coli using the GenSmartTM Codon Optimization Tool. The forbidden restriction enzymes sites were the following: BioBrick forbidden restriction sites (EcoRI, XbaI, SpeI, PstI) and Type IIS forbidden restriction sites (BsaI, SapI and Bpil). BglBrick prefix (nt 1525-1560) and suffix (nt 2277-2318) from pBbB7a (Addgene #35358) were added to the optimised sequence generated. They were designed to be cloned with Gibson Assembly.
Source
Plasmids used were from BglBrick repository. pBbB7a-GFP was a gift from Jay Keasling (Addgene plasmid # 35358 ; http://n2t.net/addgene:35358 ; RRID:Addgene_35358) [1].
References