Intermediate

Part:BBa_K133011

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)
Revision as of 21:19, 29 October 2008 by Ancksha (Talk | contribs)

CF213-UreB-linker

CF213 – the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215) In combination with CF215 it forms a TLR5 activating protein.

UreB is a beta subunit of H. pylori urease that we used as an antigen.

Linker enables easier and more correct folding of the protein.

This part is an intermediate to [1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
    Illegal AgeI site found at 877
    Illegal AgeI site found at 2055
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 763


[edit]
Categories
Parameters
None