Part:BBa_K3463018:Experience

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Characterization of BBa_K3463018

To validate the UTR designer prediction and check the B0017 relevance, we measured the fluorescence and the absorbance of the construction PtacHigh-GFP, PtacLow-GFP, PtacHigh-GFP-B0017, PtacLow-GFP-B0017, and a non-transformed strain as a negative control for to 4-5h

Figure 1 Effect of UTR prediction and B0017 terminator on eGFP fluorescence expression. The Figure 5 shows that no fluorescence was detected in the culture of the control non- transformed strain. As predicted by UTR designer the strong 5’UTR sequence (high) gave higher fluorescent levels than the weak 5’UTR sequence (Low). The terminator B0017 seems to have a limited effect on the fluorescent levels. The last seems to only impact the eGFP production of the strong UTR sequence.

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