Part:BBa_K3562011

CXCL2

Growth-regulated beta protein (GRO-beta) which produced by activated monocytes and neutrophils and shows a highly enhanced hematopoietic activity.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

Chemokines are a family of small chemotactic cytokines, and the name is derived from their ability to induce directed chemotaxis in nearby responsive cells. All chemokines possess a number of conserved cysteine residues involved in intramolecular disulfide bond formation. Some chemokines can be induced during an immune response to recruit cells of the immune system to a site of infection, while others are considered homeostatic and are involved in controlling the migration of cells during normal processes of tissue maintenance or development. Chemokines are found in all vertebrates, some viruses and some bacteria.

GRO-beta/CXCL2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. It is a hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.

Results

We used the Transwell experiment(chamber diameter: 6.5 mm, Pore diameter of PC membrane: 8 μm, 48 pores/board) combined with flow cytometry to verify the chemotactic ability of this chemokine on the THP1 cell line.(Fig.1)

Figure 1 Chemotaxis of CXCL2 to THP1(shown by cell number). Chemokine concentration is 10 nmol/l.(n=3)

Besides, we also tested the activity of six other chemokines.(Fig.2)

Figure 2 Chemotaxis index(CI) of 7 chemokines to THP1.(n=3)

Reference

[1]Chemokine activity definition
[2]UniProtKB - P19875 (CXCL2_HUMAN)