Part:BBa_K133009

CF213-UB33-CF215-RGD-Histop

This part composes of two parts of chimeric flagellin, between which we have put an urease epitope:

CF213 is the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)

UB33 is a peptide derived from the catalytic site of beta subunit of H. pylori urease. Urease is an important pathogenicity factor for H. pylori since it enables the bacteria to breakdown of urea into ammonia and carbon dioxide, thus artificially buffering the acidic enviroment in the stomach.

CF215 is the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coli flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1376
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 718
  Illegal BamHI site found at 933
  Illegal BamHI site found at 1444
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 301
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1727