Composite
Part:BBa_K3606100
Designed by: Yuanyuan Liu Group: iGEM20_Fudan (2020-10-23)
Revision as of 13:31, 26 October 2020 by Zhenru-Zhang (Talk | contribs)
J23106-RBS-luxI-B0015-J23106-RBS-luxR
J23106-RBS-luxI-B0015-luxR
Usage and Biology
luxpR_CaAP is a composite part constructed to correlate the quorum-sensing module and the calcium absorption module of our project.The promoter luxpR is activated by the heterodimer of luxI and luxR. When the population of our bacteria is low,the amount of the heterodimer is not enough, thus the luxpR promoter cannot initiate expression of downstream genes.
When the population of bacteria reaches a certain level, the heterodimer triggers the luxpR promoter, so that our engineered probiotics will express and secrete CaAP and play a role in facilitating calcium absorption.
See more information about luxI-luxR-luxpR regulating system on BBa_K3606100.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 701
Illegal EcoRI site found at 1074 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1074
Illegal NheI site found at 11
Illegal NheI site found at 34
Illegal NheI site found at 1096
Illegal NheI site found at 1119
Illegal SpeI site found at 702
Illegal PstI site found at 716
Illegal NotI site found at 709 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1074
Illegal BglII site found at 694 - 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 702
Illegal EcoRI site found at 1074 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1074
Illegal SpeI site found at 702
Illegal PstI site found at 716 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |