Plasmid_Backbone
Transfecti

Part:BBa_K157041:Design

Designed by: iGEM Team Freiburg 2008   Group: iGEM08_Freiburg   (2008-10-26)
Revision as of 21:04, 29 October 2008 by Moritz (Talk | contribs) (→‎Design Notes)

Transfection vector (similar to pSB1AK8)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 3405
    Illegal XbaI site found at 3420
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XhoI site found at 1272
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2444


Design Notes

CMV Promotor is a viral regulatory element to mediate strong expression of the downstream gene sequence. CMV stands for cytomegalovirus. Because of its strong activity in most cells its a very popular choice to mediate expression of CFP.

Source

Derived by cloning of the parts BBa_K157040 and BBa_I757009.

References