Part:BBa_K3562012

CXCL3

Growth-regulated gamma protein(GRO-gamma) that has chemotactic activity for neutrophils.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

Chemokines are a family of small chemotactic cytokines, and the name is derived from their ability to induce directed chemotaxis in nearby responsive cells. All chemokines possess a number of conserved cysteine residues involved in intramolecular disulfide bond formation. Some chemokines can be induced during an immune response to recruit cells of the immune system to a site of infection, while others are considered homeostatic and are involved in controlling the migration of cells during normal processes of tissue maintenance or development. Chemokines are found in all vertebrates, some viruses and some bacteria.

GRO-gamma/CXCL3Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.

In our project, chemokines are used to attract immune cells to accumulate to the lesion faster, replacing the original role of quorum sensing factors in the immune response.

Results

We used the Transwell experiment combined with flow cytometry to verify the chemotactic ability of this chemokine on the THP1 cell line.(Fig.1)

Figure 1 Chemotaxis of CXCL3 to THP1(shown by cell number). Chemokine concentration is 10 nmol/l.(n=3)

Besides, we also tested the activity of six other chemokines.(Fig.2)

Figure 2 Chemotaxis index(CI) of 7 chemokines to THP1.(n=3)

Reference

[1]Chemokine activity definition
[2]UniProtKB - P19876 (CXCL3_HUMAN)