Coding

Part:BBa_K3452010:Design

Designed by: Hao Zhou   Group: iGEM20_CSU_CHINA   (2020-10-26)
Revision as of 11:38, 26 October 2020 by Howie Chow (Talk | contribs)


TMCd1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 495
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization is needed so that it can be expressed successfully in Synechocystissp.

Result

EDT

Source

Tetrahymena sp. RN-2007

References

Suleman A, Shakoori AR. Evaluation of physiological importance of metallothionein protein expressed by Tetrahymena cadmium metallothionein 1 (TMCd1) gene in Escherichia coli. J Cell Biochem. 2012;113(5):1616–1622. doi:10.1002/jcb.24030