Part:BBa_K3351005

HD5d5, an antimicrobial peptide.

Summary

A human antimicrobial peptide with 32 amino acids.A simplified derivative of HD5 constructed by disulfide reduction and Arg introduction, is able to bind to and penetrate MDRAb membranes, causing cell death by catastrophic collapse and metabolic disruption. In addition to the contribution to electrostatic interactions, Arg also benefits peptides by inducing negative Gaussian curvature and pore formation on bacterial membranes. And HD5d5 enters cytoplasm, where it interacts with cellular contents and decreased the activities of SOD and catalase, resulting in an accumulation of ROS in the cells. Due to the correlation with metalloenzyme inactivation and DNA destruction, the overload of ROS is responsible for the elimination of E. coli and A. baumannii by many antibiotics.

Characterization

Figure.1 growth curve

Reference

[1] Wang C, Zhao G, Wang S, Chen Y, Gong Y, Chen S, Xu Y, Hu M, Wang X, Zeng H, Wang A, Liu D, Su Y, Cheng T, Chen F, Wang J. A Simplified Derivative of Human Defensin 5 with Potent and Efficient Activity against Multidrug-Resistant Acinetobacter baumannii. Antimicrob Agents Chemother. 2018 Jan 25;62(2):e01504-17. doi: 10.1128/AAC.01504-17. PMID: 29158275; PMCID: PMC5786806.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]