Reporter

Part:BBa_K3505018

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-20)
Revision as of 10:34, 26 October 2020 by Venetios (Talk | contribs) (→‎Design Notes)


sfGFP GB compatible with B3-B5


Level 0 sfGFP

Fig.1:sfGFP


Fig.2:sfGFP uder control of pTRC

Usage and Biology

A reporter gene fluorescent protein

  • Excitation at 485 nm
  • Emission at 510 nm


Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 149
  • 1000
    COMPATIBLE WITH RFC[1000]

[edit]
Categories
Parameters
None