Part:BBa_K3338003

P2A self-cleaving peptide without GSG-linker

Usage and Biology

The P2A peptide described here, is a self-cleaving peptide consisting of 18 aa that originates from the porcine teschovirus-1. It belongs to the group of 2A-peptides which is widely distributed among viruses. In viruses they are used to generate so called polyproteins in which the single proteins are interspaced with self-cleaving linkers (Luke et al. 2008). Although it is named a “self-cleaving” peptide the peptide bond between P and G on its C-terminal end is not cleaved after translation. It is rather believed that the ribosome fails to close the appropriate peptide bond but after that proceeds with translation (Sharma et al. 2012). In molecular biology it is used to generate polycistronic expression vectors (Szymczak-Workman et al. 2012).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Luke, G. A., de Felipe, P., Lukashev, A., Kallioinen, S. E., Bruno, E. A., & Ryan, M. D. (2008). Occurrence, function and evolutionary origins of '2A-like' sequences in virus genomes. The Journal of general virology, 89(Pt 4), 1036–1042.

Sharma, P., Yan, F., Doronina, V. A., Escuin-Ordinas, H., Ryan, M. D., & Brown, J. D. (2012). 2A peptides provide distinct solutions to driving stop-carry on translational recoding. Nucleic acids research, 40(7), 3143–3151.

Szymczak-Workman, A. L., Vignali, K. M., & Vignali, D. A. (2012). Design and construction of 2A peptide-linked multicistronic vectors. Cold Spring Harbor protocols, 2012(2), 199–204.