Part:BBa_K3521001

FnCpf1

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) systems are adaptive immune systems widely distributed across bacteria and archaea, designed to destroy DNA of invading mobile genetic elements. These immune systems, in which endonucleases are guided by single stranded RNA to find their target DNA, have been turned into powerful genome editing tools over the past few years FnCpf1 is one of class II family of CRISPR-Cas RNA programmable endonucleases with unique functions. In this project, FnCpf1 can mediate RNA-guided DNA cleavage and target single-stranded DNA to produce fluorescence.

Cpf1

Usage and Biology

Our new part is used to detect the presence of Listeria monocytogenes, which is easily survival in low temperature like refrigerator environment,and then cause Listeriosis illness, like fever, muscle ache, and so on.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1717
  Illegal NheI site found at 3437
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 3498
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]