Protein_Domain

Part:BBa_K3582023

Designed by: Avadhoot Jadhav   Group: iGEM20_IISER-Pune-India   (2020-10-20)
Revision as of 07:33, 26 October 2020 by Avadhoot (Talk | contribs)

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Cyclotide Kalata B1 domain with strep II tag

The biobrick codes for the cyclotide kalata B1 protein domain consisting of the first 5 loops of the cyclotide construct. This part has been created to allow insertion of peptide sequences in the 6th loop.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 29

References

  • 1] Cyclotides, a novel ultrastable polypeptide scaffold for drug discovery. (2011, December 1). PubMed

Central (PMC). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330703/

  • 2] Poth, A. G. (2013, May 7). Cyclotides as Grafting Frameworks for Protein Engineering and Drug Design

Applications. PubMed. https://pubmed.ncbi.nlm.nih.gov/23893608/

  • 3] 2. Camarero, J. A. (2019, April 19). The Potential of the Cyclotide Scaffold for Drug Development.

MDPI. https://www.mdpi.com/2227-9059/7/2/31/htm#B31-biomedicines-07-00031


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Categories
Parameters
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