Protein_Domain

Part:BBa_K3582004

Designed by: Saillesh Aravindhan C   Group: iGEM20_IISER-Pune-India   (2020-10-20)
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The DBLb domain of PF11_0521 PfEMP1

This biobrick is thought to be able to produce the DBLb domain of PfEMP-1. Plasmodium falciparum erythrocyte membrane protein 1 EMP1 is encoded by the clonally variant var gene family with approximately 60 members per parasite genome. The adhesion process of the PfEMP1 proteins is often described as a way for the parasite to evade splenic clearance and has also been reported as associated with vascular obstruction of vital organs leading to major pathological events.[1]. These proteins are also known for their ability to mediate adhesion of IEs to host receptors in the vasculature.[2]

The binding of PfEMP1 to ICAM-1 is mediated by a subset of Duffy binding-like (DBL) domains, the DBLβ domains, which are found in either A-type or BC-type PfEMP1.[4][5][6][7]. The PfEMP1 protein to the D1 domain of ICAM-1[3].

Figure 1.Structure of the DBLb domain of PF11_0521 PfEMP1 protein visualized using Chimera


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1407
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1113
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  • [1]Baratin, M., Roetynck, S., Pouvelle, B., Lemmers, C., Viebig, N. K., Johansson, S., Bierling, P., Scherf, A., Gysin, J., Vivier, E., & Ugolini, S. (2007). Dissection of the role of PfEMP1 and ICAM-1 in the sensing of Plasmodium-falciparum-infected erythrocytes by natural killer cells. PloS one, 2(2), e228. https://doi.org/10.1371/journal.pone.0000228
  • [2]Hviid L, Jensen AT. PfEMP1 ‐ A parasite protein family of key importance in Plasmodium falciparum malaria immunity and pathogenesis. Adv Parasitol. 2015;88:51‐84
  • [3]Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA. Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Cell. 1988;52:925–933.
  • [4]A. Bengtsson et a., A novel domain cassette identifies Plasmodium falciparum PfEMP1 proteins binding ICAM-1 and is a target of cross-reactive, adhesion-inhibitory antibodies. J. Immunol. 190, 240–249 (2013).
  • [5]E. Carrington et al., In silico guided reconstruction and analysis of ICAM-1-binding var genes from Plasmodium falciparum. Sci. Rep. 8, 3282 (2018)
  • [6] D. P. G. Howell et al., Mapping a common interaction site used by Plasmodium falciparum Duffy binding-like domains to bind diverse host receptors. Mol. Microbiol. 67, 78–87 (2008).CrossRefPubMedGoogle Scholar
  • [7] H. Janes et al., Investigating the host binding signature on the Plasmodium falciparum PfEMP1 protein family. PLoS Pathog. 7, e1002032 (2011).


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