Coding
LacY

Part:BBa_K079015:Design

Designed by: Francesca Ceroni   Group: iGEM08_Bologna   (2008-10-24)
Revision as of 20:17, 29 October 2008 by Mavi.amaduzzi (Talk | contribs) (→‎Design Notes)

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LacY transporter protein from E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 757
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Derived from the BBa_J22101 part

References

Hansen et al. "The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens". (Current Microbiology, 1998)

Abramson et al. "The lactose permease of Escherichia coli : overall structure,the sugar-binding site and the alternating access model for transport". (FEBS Letters, 2003)

Mirzna et al. "Structural evidence for induced fit and a mechanism for sugar/Hþ symport in LacY". (The EMBO Journal, 2006)