Part:BBa_K3629004

Yarrowia lipolytica XPR2 terminator

Terminator sequence from the Yarrowia lipolytica alkaline extracellular protease XRP2 gene.

Usage and Biology

XPR2 terminator is a native Yarrowia lipolytica terminator for the alkaline XPR2 exonulcease gene. XPR2 is among the most common terminators used for heterologous expression of proteins in Y. lipolytica. (1)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 271
  Illegal EcoRI site found at 265
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 265
  Illegal SpeI site found at 272
  Illegal PstI site found at 286
  Illegal NotI site found at 7
  Illegal NotI site found at 279
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 265
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 272
  Illegal EcoRI site found at 265
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1
  Illegal EcoRI site found at 265
  Illegal XbaI site found at 16
  Illegal SpeI site found at 272
  Illegal PstI site found at 286
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Czajka, Jeffrey J, Nathenson, Justin A, Benites, Veronica T, Baidoo, Edward E. K, Cheng, Qianshun, Wang, Yechun, & Tang, Yinjie J. (2018). Engineering the oleaginous yeast Yarrowia lipolytica to produce the aroma compound β-ionone. Microbial Cell Factories, 17(1), 136–136. https://doi.org/10.1186/s12934-018-0984-x