Regulatory

Part:BBa_K3629001

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-09)
Revision as of 06:12, 26 October 2020 by ArshiaMostoufi (Talk | contribs) (→‎Usage and Biology)


Yarrowia lipolytica TEF1 promoter with an intron

Promoter from the Yarrowia lipolytica Translation Elongation Factor 1 (TEF1) gene with the first intron.

Usage and Biology

This is the natural promoter found in the wild-type Yarrowia lipolytica for the Translation Elongation Factor 1 (TEF1) gene. TEF promoter is among the strongest constitutive promoters found in Yarrowia lipolytica. Inclusion of the first intron of the TEF1 gene in the promoter sequence has shown increase the expression levels. It can be placed immediately upstream of a coding sequence for Y. lipolytica.

Design

hellllloooooo where is this showing up

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

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[edit]
Categories
//awards/part_collection
//chassis/eukaryote/yeast
Parameters
None