Composite

Part:BBa_K3365054:Design

Designed by: Zou Aixuan   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-22)
Revision as of 06:11, 26 October 2020 by Gyaxxxxxx (Talk | contribs)


enhanced GFP with double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 721
    Illegal XhoI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We get double terminator from plasmid pE1a-RFP kindly offered by team Tongji_China and get eGFP from plasmid HGT.


Source

Due to complex secondary structure of double terminator, it is quite hard to add double terminator with eGFP fragment only by performing PCR twice.

References