Composite

Part:BBa_K3624007:Design

Designed by: Emily Hagens   Group: iGEM20_Lethbridge   (2020-10-26)
Revision as of 05:08, 26 October 2020 by Emily Hagens (Talk | contribs) (→‎References)

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Tu-AMP2: Antifungal peptide.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 85
    Illegal XbaI site found at 100
    Illegal SpeI site found at 172
    Illegal PstI site found at 186
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 85
    Illegal SpeI site found at 172
    Illegal PstI site found at 186
    Illegal NotI site found at 91
    Illegal NotI site found at 179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 85
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 85
    Illegal suffix found in sequence at 172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 85
    Illegal XbaI site found at 100
    Illegal SpeI site found at 172
    Illegal PstI site found at 186
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence was codon-optimized.


Source

Tu-AMP2 comes from Tulip Bulbs (Tulipa gesneriana).

References

Fujimura, M., Ideguchi, M., Minami, Y., Watanabe, K., & Tadera, K. (2004). Purification, Characterization, and Sequencing of Novel Antimicrobial Peptides,Tu-AMP 1 andTu-AMP 2, from Bulbs of Tulip (Tulipa gesnerianaL.). Bioscience, Biotechnology, and Biochemistry, 68(3), 571-577. doi:10.1271/bbb.68.571