Coding

Part:BBa_K3624004:Design

Designed by: Emily Hagens   Group: iGEM20_Lethbridge   (2020-10-25)
Revision as of 04:51, 26 October 2020 by Emily Hagens (Talk | contribs) (→‎References)


Snakin-2: Antibacterial and antifungal peptide.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 226
    Illegal PstI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 226
    Illegal PstI site found at 240
    Illegal NotI site found at 7
    Illegal NotI site found at 233
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 226
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 226
    Illegal PstI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence was codon-optimized.


Source

Snakin-2 comes from potatoes (Solanum tuberosum).

AMP Database ID: AP00992

References

Wang, G. (2020). The Antimicrobial Peptide Database. Retrieved 2020