Plasmid

Part:BBa_K165060

Designed by: Aaron Glieberman   Group: iGEM08_BrownTwo   (2008-10-29)
Revision as of 19:47, 29 October 2008 by Registry (Talk | contribs)

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pRS303 yeast shuttle vector, HIS3

Useful for chromosomal integration of a part into yeast strains.

R Daniel Gietz & Robert H Schiestl. High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method . Nature protocols


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2127
    Illegal XbaI site found at 2097
    Illegal SpeI site found at 2103
    Illegal PstI site found at 1182
    Illegal PstI site found at 2121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2127
    Illegal NheI site found at 1007
    Illegal SpeI site found at 2103
    Illegal PstI site found at 1182
    Illegal PstI site found at 2121
    Illegal NotI site found at 2089
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2127
    Illegal BglII site found at 898
    Illegal BglII site found at 958
    Illegal BamHI site found at 2109
    Illegal XhoI site found at 2160
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2127
    Illegal XbaI site found at 2097
    Illegal SpeI site found at 2103
    Illegal PstI site found at 1182
    Illegal PstI site found at 2121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2127
    Illegal XbaI site found at 2097
    Illegal SpeI site found at 2103
    Illegal PstI site found at 1182
    Illegal PstI site found at 2121
    Illegal NgoMIV site found at 1748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3533
    Illegal SapI site found at 2450


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