Part:BBa_K3562002

pLasRV

las promoter from Pseudomonas aeruginosa

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 14
  

Usage and Biology

We found this well-characterized promoter pLasRV from P. aeruginosa cloned by Paul Freemont group in Imperial College, with in vivo and in vitro expression data thoroughly presented in the research articles [1]. Notably, researchers developed a cell-free biosensor based on this promoter to detect the concentration of OdDHL in sputum samples [2]. More attempts to work on this inducible promoter in iGEM will deepen the understanding of quorum sensing in P. aeruginosa and facilitate the exploitation of biomarker-based biosensors.

Figure 1 Characterization of inducible promoter pLasRV in cell-free systems and E. coli [1]

Figure 2 Overview of the LasRV cell-free biosensor [2]

Figure 3 Detection of sputum sample using the LasRV cell-free biosensor [2]

Reference

[1]Chappel J et al., Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Research, 41 (2013): 3471–3481. [2]Wen KY et al., A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples. ACS Synth. Biol., 6 (2017): 2293-2301.