Composite

Part:BBa_K118024

Designed by: Andrew Hall   Group: iGEM08_Edinburgh   (2008-10-07)
Revision as of 19:24, 29 October 2008 by Cfrench (Talk | contribs)

dxs+LIMS1+appY

dxs, from Escherichia coli JM109, encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses the first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here]. lims1, from Citrus limon, encodes limonene synthase, which produces (+)-limonene from farnesyl diphosphate (an intermediate in the mevalonate pathway)(Lucker, J., El Tamer, M.K., Schwab, W., Verstappen, F.W.A., van der Plas, L.H.W., Bouwmeester, H.J., and Verhoeven, H.H. 2002. Monoterpene biosynthesis in lemon (Citrus limon). European Journal of Biochemistry 269, 3160-3171). (+)-Limonene is a component of lemon scent. appY, from Escherichia coli JM109, encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of the gene has been reported to result in enhanced lycopene production by E. coli (Kang et al., 2005). (All three genes are individually preceded by a ribosome binding site.)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 746
    Illegal SapI.rc site found at 2006


[edit]
Categories
Parameters
None