Part:BBa_K3362003
Transcriptional Synchronization Cassette (Eukaryotic Version)
The eukaryote plant cassette consists of mCherry, a poly-A tail, HDVRz, HHRz, sense YFP and antisense YFP. mCherry is acting as reporter. HDVRz inhibits the attachment of a functional poly-A tail, therefore an artificial one was added to the sequence. HHRz is cutting at the 3'-end of the sense YFP and at the 5'-end of the antisense YFP. The connection between sense YFP and antisense YFP is formed by a spacer inheriting a loop, so after the cleavage a double strand is formed. The plant itself possesses a mechanism to destroy the loop, thus breaking the previously formed double strand. The antisense strand can then be used by the RNAi machinery for further silencing processes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 50
Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 1695
Illegal NgoMIV site found at 1724 - 1000COMPATIBLE WITH RFC[1000]
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