Part:BBa_K3589211

Level 2 � spectinomycin resistance + cCA_BaLac_HA-RGS-8His

This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant laccase from Botrytis aclada (BBa_K3589108) fused with a HA-RGS-8His-tag (BBa_K3589151) for easy detection via HA-antibody and purification via nickel-NTA-column.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2401
  Illegal PstI site found at 3586
  Illegal PstI site found at 3673
  Illegal PstI site found at 3737
  Illegal PstI site found at 4109
  Illegal PstI site found at 4397
  Illegal PstI site found at 4474
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2401
  Illegal NheI site found at 2665
  Illegal PstI site found at 3586
  Illegal PstI site found at 3673
  Illegal PstI site found at 3737
  Illegal PstI site found at 4109
  Illegal PstI site found at 4397
  Illegal PstI site found at 4474
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2401
  Illegal BglII site found at 4033
  Illegal BglII site found at 4992
  Illegal BamHI site found at 4372
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2401
  Illegal PstI site found at 3586
  Illegal PstI site found at 3673
  Illegal PstI site found at 3737
  Illegal PstI site found at 4109
  Illegal PstI site found at 4397
  Illegal PstI site found at 4474
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2401
  Illegal PstI site found at 3586
  Illegal PstI site found at 3673
  Illegal PstI site found at 3737
  Illegal PstI site found at 4109
  Illegal PstI site found at 4397
  Illegal PstI site found at 4474
  Illegal NgoMIV site found at 1401
  Illegal NgoMIV site found at 1584
  Illegal NgoMIV site found at 1694
  Illegal NgoMIV site found at 6106
  
• 1000
  COMPATIBLE WITH RFC[1000]