Part:BBa_K3696005

A topological DNA sequence that can bind to T7 promoter and inhibit transcription.

BBa_K3696004--BBa_K3696007 are used in TO-DNA testings, the protocol is shown below:

TO-DNA protocol

TO-DNA system protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

Key strand concentration testing

From the detected fluorescence, we determine that Key1 OR-IN1 is more effective than Key1 OR-IN2, and its limit for detection is 1 nM.

Key strand 1 complementary sequence testing

However, we find that the reaction might start earlier than expected after combining TO-DNA system to DNAzyme, so we decide to give up this system.

Sequence and Features

Assembly Compatibility: