DNA

Part:BBa_K3696003

Designed by: Yuanhong Pan   Group: iGEM20_OSA   (2020-10-25)
Revision as of 16:06, 25 October 2020 by Hpan20 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


A short DNA sequence that can bind to 8-17 DNAzyme and 8-17 substrate strand.

BBa_K3696000, BBa_K3696002--BBa_K3696003 are used in HTDC testings, the protocol is shown below:

HTDC protocol


The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

HTDC Pb2+ testing

From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None