Coding

Part:BBa_K3562000

Designed by: Haoyu Zheng   Group: iGEM20_WHU-China   (2020-10-22)
Revision as of 16:05, 25 October 2020 by Carotene (Talk | contribs)


AiiC (with His-tag)

AHL-lactonase with AHLs (Quorum-sensing factor Acyl Homoserine Lactone) degradation activity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2071
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2071
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2071
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2071
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

AHL-lactonase is a kind of lactonase opened the homoserine lactone ring of AHL in the presence of water to produce N-acly homoserine.(Fig.1) The biological activity of degradation products will be greatly reduced.

Figure 1:Mechanism of AHL-lactonase

Quorum sensing factor is closely related to bacterial virulence. Enzymes that degrade AHL can control the concentration of AHL to silence the expression of related genes. In our project, we use these enzymes to control the virulence of Pseudomonas aeruginosa referred to existing experimental therapies.

Different AHL degrading enzymes have different substrate preferences and degradation activities. We try to select suitable ones from a variety of enzymes.

[edit]
Categories
Parameters
None