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Part:BBa_K3429003

Designed by: Jan-Philip Kahl   Group: iGEM20_TU_Darmstadt   (2020-09-19)
Revision as of 15:40, 25 October 2020 by Mehryad (Talk | contribs)


Protein Linker for fusion proteins: ARGGGGSGGGGSGS


We use this flexible glycine-serine linker (GGGGSGGGGS) to create TasA fusion proteins with CotA, CuO and EreB. Huang et al fused TasA to superfolder GFP by using this protein linker without impairing TasA or sfGFP function. [1]

References

[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None