DNA

Part:BBa_K3696001

Designed by: Yuanhong Pan   Group: iGEM20_OSA   (2020-10-25)
Revision as of 14:56, 25 October 2020 by Hpan20 (Talk | contribs)


A type of DNA oligonucleotide that can cleave towards its substrate after binding to Pb2+.

BBa_K3696001, BBa_K3696002 are used in 8-17 DNAzyme testings, the protocol is shown below:
8-17 DNAzyme protocol
The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

8-17 DNAzyme(two parts) Pb2+ testing

From the detected fluorescence, we determine that: 8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None