Coding

Part:BBa_K3429002

Designed by: Jan Lars Kalkowski   Group: iGEM20_TU_Darmstadt   (2020-09-19)
Revision as of 14:47, 25 October 2020 by Mehryad (Talk | contribs)


Superfolder Green Fluorescent Protein (sfGFP)


The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein. [1] We implement it as a reporter and experimental control for our TasA fusion proteins with the enzymes CotA, CuO and EreB.


References

[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None