Device
Part:BBa_K3402059
Designed by: Yibo Shi Group: iGEM20_Jiangnan_China (2020-10-25)
Cas9 expression cassette
This device is composed of upSBLE (BBa_K3402030), Ptef1 (BBa_K3402007), Cas9 (BBa_K3402023), NLS (BBa_K3402008), Tsyn7 (BBa_K3402001) and doSBLE (BBa_K3402031).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2078
Illegal BglII site found at 5642
Illegal XhoI site found at 1399
Illegal XhoI site found at 2128
Illegal XhoI site found at 2491
Illegal XhoI site found at 5443 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2905
Illegal NgoMIV site found at 4009 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1849
Illegal BsaI site found at 2566
[edit]
Categories
Parameters
None |