Device

Part:BBa_K3402059

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-25)
Revision as of 14:39, 25 October 2020 by Registry (Talk | contribs)

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Cas9 expression cassette

This device is composed of upSBLE (BBa_K3402030), Ptef1 (BBa_K3402007), Cas9 (BBa_K3402023), NLS (BBa_K3402008), Tsyn7 (BBa_K3402001) and doSBLE (BBa_K3402031).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2078
    Illegal BglII site found at 5642
    Illegal XhoI site found at 1399
    Illegal XhoI site found at 2128
    Illegal XhoI site found at 2491
    Illegal XhoI site found at 5443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2905
    Illegal NgoMIV site found at 4009
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1849
    Illegal BsaI site found at 2566


[edit]
Categories
Parameters
None