RNA

Part:BBa_K3657022

Designed by: Julia Langer   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 14:09, 25 October 2020 by Liza ch (Talk | contribs)


RNA Part of an RNA·DNA-DNA triple helix

!!!This part works in combination with the part BBa_K3657023 .!!!

It was proved by Knucker et al. that the transcript of this sequence creates a triple helix with the DNA part (BBa_K3657023) in vitro.

More information available here: LINK

You can generate analogical parts yourself as following:

  1. Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.
    2. <liUse the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None