RNA

Part:BBa_K3657022

Designed by: Julia Langer   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 14:07, 25 October 2020 by Liza ch (Talk | contribs)


RNA Part of an RNA·DNA-DNA triple helix

!!!This part works in combination with the part BBa_K3657023 .!!!

It was proved by Knucker et al. that the transcript of this sequence creates a triple helix with the DNA part (BBa_K3657023) in vitro.

More information available here: LINK

You can generate analogical parts yourself as following: 1. Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine. 2. Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None