Part:BBa_K3429001

TasA matrix protein

TasA protein

The TasA protein (also known as the major biofilm matrix component) is essential for biofilm structures and polymerizes into long fibers attached to the B. subtilis cells. ^{<a href="#cite_ref-1">[1</a>,}^{<a href="#cite_ref-2">2]</a>}

We designed TasA fusion proteins with CotA, CuO and EreB to immobilize our degrading enzymes in the biofilm matrix. Protein secretion into the biofilm matrix is regulated by the Sec-dependent signal recognition partice (sec-SRP) pathway.

                           <a href="#cite_note-3">[3]
                            </a>

References

<a href="#cite_ref-1">↑</a> Branda, S.; Chu, F.; Kearns, D. (2006): A major protein component of the Bacillus subtilis biofilm matrix. In: Molecular microbiology 59 (4), S. 1229–1238, DOI 10.1111/j.1365-2958.2005.05020.x. <a rel="nofollow" class="external autonumber" href="https://doi.org/10.1111/j.1365-2958.2005.05020.x.">[3] </a>
<a href="#cite_ref-2">↑</a> Romero, D.; Aguilar, C.; Losick, R. (2010): Amyloid fibers provide structural integrity to Bacillus subtilis biofilms. In: Proceedings of the National Academy of Sciences of the United States of America 107 (5), S. 2230–2234,DOI 10.1073/pnas.0910560107x. <a rel="nofollow" class="external autonumber" href="https://doi.org/10.1073/pnas.0910560107x.">[2] </a>
<a href="#cite_ref-3">↑</a> Stöver, A.; Driks, A. (1999): Secretion, Localization, and Antibacterial Activity of TasA, a Bacillus subtilis Spore-Associated Protein. In: Journal of Bacteriology 181 (5), S. 1664–1672. <a rel="nofollow" class="external autonumber" href="https://doi.org/10.1111/j.1365-2958.2005.05020.x.">[3] </a>

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

[1] <a href="#cite_ref-1">↑</a> Branda, S.; Chu, F.; Kearns, D. (2006): A major protein component of the Bacillus subtilis biofilm matrix. In: Molecular microbiology 59 (4), S. 1229–1238, DOI 10.1111/j.1365-2958.2005.05020.x. <a rel="nofollow" class="external autonumber" href="https://doi.org/10.1111/j.1365-2958.2005.05020.x.">[3] </a>

[2] <a href="#cite_ref-2">↑</a> Romero, D.; Aguilar, C.; Losick, R. (2010): Amyloid fibers provide structural integrity to Bacillus subtilis biofilms. In: Proceedings of the National Academy of Sciences of the United States of America 107 (5), S. 2230–2234,DOI 10.1073/pnas.0910560107x. <a rel="nofollow" class="external autonumber" href="https://doi.org/10.1073/pnas.0910560107x.">[2] </a>

[3] <a href="#cite_ref-3">↑</a> Stöver, A.; Driks, A. (1999): Secretion, Localization, and Antibacterial Activity of TasA, a Bacillus subtilis Spore-Associated Protein. In: Journal of Bacteriology 181 (5), S. 1664–1672. <a rel="nofollow" class="external autonumber" href="https://doi.org/10.1111/j.1365-2958.2005.05020.x.">[3] </a>