Part:BBa_K3589108

Mutant laccase from Botrytis aclada for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the coding sequence of the laccase from the ascomycete Botrytis aclada mutant L499F (B3 – B4). This part is codon-optimized for Chlamydomonas reinhardtii. Combined with a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. As this part contains the introns 1 – 3 of RBCS2, it perfectly matches the part BBa_K3002027 (pAR promoter A1-B2), resulting in a high expression (Eichler-Stahlberg et al., 2009). To detect the target protein a 3xHA-tag from the Kaiser Collection (BBa_K3002017) is recommended. We recommend using the pAR promoter (A1-A3) (BBa_K3002003).
This part was designed to allow synthesis due to a high GC-level.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 608
  Illegal PstI site found at 695
  Illegal PstI site found at 759
  Illegal PstI site found at 1131
  Illegal PstI site found at 1419
  Illegal PstI site found at 1496
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 608
  Illegal PstI site found at 695
  Illegal PstI site found at 759
  Illegal PstI site found at 1131
  Illegal PstI site found at 1419
  Illegal PstI site found at 1496
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1055
  Illegal BglII site found at 2014
  Illegal BamHI site found at 1394
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 608
  Illegal PstI site found at 695
  Illegal PstI site found at 759
  Illegal PstI site found at 1131
  Illegal PstI site found at 1419
  Illegal PstI site found at 1496
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 608
  Illegal PstI site found at 695
  Illegal PstI site found at 759
  Illegal PstI site found at 1131
  Illegal PstI site found at 1419
  Illegal PstI site found at 1496
  
• 1000
  COMPATIBLE WITH RFC[1000]