Part:BBa_K3589105

Mutant laccase from Botrytis aclada for Escherichia coli

This basic part contains the coding sequence of the mutant form L499F of the laccase from the ascomycete Botrytis aclada (hereafter referred to as BaLac). This part is codon-optimized for Escherichia coli. Combined a promoter and a terminator, this level 0 part should mediates the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. This part can be cloned into E coli. expression vectors like the commercially available <a href="https://www.snapgene.com/resources/plasmid-files/?set=pgex_vectors_%28ge_healthcare%29&plasmid=pGEX-6P-1">pGEX-6P-1 vector</a>. The mutant laccase L499F has a high redox potential and shows activity near neutral pH (Scheiblbrandner et. al, 2017).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 775
  Illegal PstI site found at 463
  Illegal PstI site found at 550
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 775
  Illegal PstI site found at 463
  Illegal PstI site found at 550
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 775
  Illegal BamHI site found at 920
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 775
  Illegal PstI site found at 463
  Illegal PstI site found at 550
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 775
  Illegal PstI site found at 463
  Illegal PstI site found at 550
  Illegal NgoMIV site found at 612
  Illegal AgeI site found at 136
  Illegal AgeI site found at 976
  Illegal AgeI site found at 1059
  Illegal AgeI site found at 1315
  
• 1000
  COMPATIBLE WITH RFC[1000]