![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Part:BBa_K3431005
op21_A Toehold Switch for miR-21 Detection
Introduction
op21_A toehold switch is a regulatory part for the downstream reporter gene. With this part, the protein expression can be controlled by the miR-21. The sequence of the toehold switch can be separated into the following 5 regions from its 5' end: TBS (trigger binding site), stem region, loop region with RBS(ribosome binding site), complimentary stem region with a start codon, and linker. Upon binding with miR-21, its hairpin structure can be opened up and the ribosomes can bind with its RBS (ribosome binding site), triggering the translation of the downstream reporter.
Model
NUPACK ANALYSIS
![](https://static.igem.org/mediawiki/parts/e/e3/T--CSMU_Taiwan--op21_A_NU.png)
VIENNA RNA PACKAGE
![](https://static.igem.org/mediawiki/parts/e/e4/T--CSMU_Taiwan--op21_A_Ve.png)
Link to our model page: https://2020.igem.org/Team:CSMU_Taiwan/Model
Experiment result
![](https://static.igem.org/mediawiki/parts/e/ea/T--CSMU_Taiwan--op21_A_%28BBa_K3431021%29.png)
References
Green, A. A., Silver, P. A., Collins, J. J., & Yin, P. (2014). Toehold switches: de-novo-designed regulators of gene expression. Cell, 159(4), 925-939. Pardee, K., Green, A. A., Takahashi, M. K., Braff, D., Lambert, G., Lee, J. W., ... & Daringer, N. M. (2016). Rapid, low-cost detection of Zika virus using programmable biomolecular components. Cell, 165(5), 1255-1266. Wang, S., Emery, N. J., & Liu, A. P. (2019). A novel synthetic toehold switch for microRNA detection in mammalian cells. ACS synthetic biology, 8(5), 1079-1088.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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