Coding

Part:BBa_K3552004

Designed by: Liu Xibei   Group: iGEM20_LINKS_China   (2020-10-25)
Revision as of 10:52, 25 October 2020 by Registry (Talk | contribs)

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hofM-hofN-hofO-hofP-hofQ(gene from DH5a)

HofM is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofN is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization, DNA catabolic process, type IV pilus biogenesis and type IV pilus-dependent motility. HofO is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofP is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Its functions include the carbon utilization and DNA catabolic process. HofQ is a DNA utilization protein which is required for the use of extracellular DNA as a nutrient. Could be the porin responsible for transport of DNA across the outer membrane. Its functions include the carbon utilization, DNA binding, protein secretion and DNA catabolic process.This part is in the part collection where we have 13 genes that code for the proteins.

HofM is a component of the IM complex that is connected to the outer membrane secretin channel formed by the hofQ and allows pilus exposure on the bacterial surface. HofN is a component in the transmembrane protein that connected to the outer membrane secretin channel formed by the hofQ. HofO is a component in the transmembrane protein that connected to the outer membrane secretin channel formed by the hofQ. The inner membrane anchored assembly platform complex is connected via hofP to the outer membrane secretin channel formed by hofQ that allows pilus to expose on the bacterial surface. The hofMNOPQ operon encodes the assembly platform complex connecting HofB ATPase with the secretin hofQ. The hofMNOPQ is the generation pathway of pilA from the inner membrane to the surface of outer membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 11
    Illegal BglII site found at 1813
    Illegal BamHI site found at 336
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1781
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1417


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