Part:BBa_K3552006

hofB-hofC

HofB is a transport protein found in bacteria and is ATP-binding and nucleotide-binding, constitute the generator of pilA. HofC is an inner membrane protein, a base for the generator of pilA. This part is in the part collection where we have 12 genes that code for the base generator of pilA.

The hofC is from the hof operon encoding the generator of major pilin, the ATPase HofB and the platform protein HofC is localized at 3 min of E.coli map. The HofB gene is a ATPase motor which localized at the base of the pilin generator system in the inner membrane, propel the assembly of pilus and it interacts with the platform protein HofC and HofM component of the inner membrane anchored assembly platform complex that also includes HofN and HofO. The hofMNOPQ operon encodes the assembly platform complex connecting HofB ATPase with the secretin hofQ.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 872
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1801
  
• 1000
  COMPATIBLE WITH RFC[1000]